Measuring the effects of diethyl phthalate microplastics on marine algae growth using dielectric spectroscopy

This paper presents the development of a dielectric spectroscopy-based method using a customized, transmission line probe, fabricated on a printed circuit board (PCB), for monitoring the effect of diethyl phthalate (DEP) microplastics on marine algae growth. Experiments were performed by exposing marine algae (Chlorella pyrenoidosa) to DEP (0-50 mg) for up to 6 days. In order to amplify the electrophysiological effects and improve the sensing, a glutaraldehyde crosslinking agent was used and encapsulated on the surface of the probe. The reflection coefficient (S) and the complex permittivity (ɛ' & ɛ″) of the Medium Under Test (MUT) were investigated in the frequency range of 30 kHz-800 MHz. Without the presence of DEP, the number of algae (10 cells/mL) and chlorophyll content (mg/L) increased at the rates of 207.73 × 10 cells/mL and 148.1 mg/L per day, respectively. After 6 days of exposing Chlorella pyrenoidosa (C. pyrenoidosa) algae to different DEP concentrations, the growth rate decreased down to -11.92 × 10 cells/mL and -19.19 mg/L (50 mg DEP), respectively. Additionally, the linearity of the relationship kept decreasing as the DEP content increased from R = 0.9716 to R = 0.1050 and from R = 0.9293 to R = 0.4961, respectively. Dielectric spectroscopy using the custom, transmission line probe, at 740 MHz, showed linear relationship (-1.22 dB/day) between the reflection coefficient (S) and hence complex permittivity (ɛ' & ɛ″) without the presence of DEP. However, as the DEP content increased, algae growth was prohibited more intensely, shown both from the number of algae and the chlorophyll content. This trend was reflected on S and subsequently on the complex permittivity. This relationship confirms the capability of this method to monitor the growth of marine algae in almost real-time. This dielectric spectroscopy method could be a potential, low-cost tool to examine the impact of microplastic pollutants on marine microorganisms.