We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Assessment of Microbial Load and Multidrug-Resistant Profile of Bacterial Flora from Cattle in Bauchi, Nigeria
Summary
Researchers isolated Enterococcus spp. and Escherichia coli from cattle rectal swabs at two farm sites in Bauchi, Nigeria, and assessed their multidrug-resistance profiles using the Kirby-Bauer disc diffusion method. Microbial loads were high (up to 3.4 x 10^12 CFU/g) and most isolates demonstrated resistance to multiple antimicrobial agents, raising food safety and antimicrobial resistance concerns.
Aim: The study aimed to assess the bacterial load of in rectal swabs from cattle by isolating Enterococcus spp and Escherichia coli, and determining the multidrug-resistant pattern of the isolates. Study Design: The study is a clinical-veterinary laboratory investigation involving the isolation and determination of the multidrug-resistant (MDR) profile of Enterococcus spp and E. coli isolated from cattle rectal. Place and Duration of Study: This study was carried out in the Yelwa and Gubi campuses Farm centers of Abubakar Tafawa Balewa University (ATBU), Bauchi, Nigeria, in period extended from April to June 2021. Methodology: Fresh rectal swab samples were collected from the randomly selected cattle and labeled. The samples were immediately transported and processed in the Microbiology laboratory at Yelwa Campus, and the bacterial load of each sample was determined using standard techniques. Enterococcus spp and E. coli were isolated using differential culture media followed by an appropriate biochemical identification test. The isolates were subjected to the Kirby-Bauer disc diffusion method, to assess the antimicrobial susceptibility pattern. Results: In Yelwa, the highest microbial load is 2.7 x 1012 CFU/g. while the lowest microbial load is 2.0 x 1012 CFU/g. In the Gubi campus, the highest microbial load is 3.4 x 1012 CFU/g. while the lowest microbial load is 2.7 x 1012 CFU/g. Both in Yelwa and Gubi ,the result showed that most isolates of Enterococcus spp and E. coli are multidrug-resistant. In Yalwa some of the isolates showed 100% resistance against Norfloxacin, Rifampicin, Ampicillin, and Streptomycin, while Gentamycin gave the lowest multidrug resistance (57.4%). In Gubi, the highest was to ampicillin with (90.6%) frequency, while the lowest resistance was found in Chloramphenicol (11.3%). In Yelwa, a high percentage resistance (92.6%) was observed in Streptomycin, and Cephalexin has the lowest (20.4%). In Gubi, all the E. coli isolates had 100% resistance against sulfamethoxazole, and the lowest was in Ofloxacin (43.4%). Conclusion: This study found that cattle in the area are reservoirs of bacteria that are both part of the normal flora and opportunistic pathogens, and harbored resistance phenotypes. It is therefore advocated that the use of these animals’ faeces as manure should be done with caution, particularly after pre-treatments.
Sign in to start a discussion.
More Papers Like This
Assessing antimicrobial and metal resistance genes in Escherichia coli from domestic groundwater supplies in rural Ireland
Researchers analyzed E. coli bacteria from private drinking wells in rural Ireland, finding that antibiotic resistance genes were present in 17% of isolates, likely arriving in groundwater via agricultural runoff rather than forming there. Importantly, no clear link was found between metal contamination and antibiotic resistance levels, suggesting livestock farming practices are the primary driver.
Multidrug-resistant Escherichia coli causing diarrhea in yak calves on the Qinghai-Tibet Plateau: phenotypic characterization, whole-genome sequencing, and pathogenicity analysis
Scientists found dangerous, drug-resistant E. coli bacteria in sick yak calves in Tibet that doesn't respond to most common antibiotics. These "superbug" strains could potentially spread to humans through the food chain or environment, making infections much harder to treat. This highlights the growing global threat of antibiotic-resistant bacteria, especially in areas where antibiotics are used heavily in livestock.
Antibiotic resistant bacteria in diverse ecological water samples are a threat to Human Food security
Researchers isolated antibiotic-resistant bacteria from three aquatic sources -- the River Ravi, a fish farm, and underground drinking water -- identifying 33 bacterial strains across genera including Enterobacteriaceae, Pseudomonas, and Staphylococcus, and finding resistance to 8 of 10 antibiotic classes with multiple antibiotic resistance indices ranging from 0.43 to 0.88.
Pathogenic Escherichia coli Strains Recovered from Selected Aquatic Resources in the Eastern Cape, South Africa, and Its Significance to Public Health
Researchers recovered pathogenic Escherichia coli strains from aquatic environments in the Eastern Cape of South Africa, finding evidence of antimicrobial resistance genes and highlighting the public health significance of waterway contamination as a pathway for resistance proliferation.
Antibiotic Resistance Mediated by Escherichia coli in Kuwait Marine Environment as Revealed through Genomic Analysis
Researchers used genomic analysis to identify antibiotic resistance genes in Escherichia coli isolated from mollusks and coastal water samples in Kuwait's marine environment. The study found that Kuwait's coastal waters, vulnerable to sewage contamination from storm outlets and waste disposal, harbor E. coli carrying multiple antibiotic resistance gene elements including integrons and plasmids.