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Development of a yeast whole-cell biocatalyst for MHET conversion into terephthalic acid and ethylene glycol

Microbial Cell Factories 2022 20 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Raphaël Loll‐Krippleber, Victoria A. Sajtovich, Michael Ferguson, Brandon Ho, Andrew R. Burns, Brandon J. Payliss, Joseph Bellissimo, Sydney Peters, Peter J. Roy, Haley D.M. Wyatt, Grant W. Brown

Summary

Researchers engineered baker's yeast to display plastic-degrading enzymes on its cell surface, demonstrating a simpler and potentially cheaper approach to breaking down PET plastic — the material used in bottles — without requiring the costly step of purifying the enzymes first.

Polymers
PET

We demonstrate the feasibility of using S. cerevisiae as a platform for the expression and surface display of PET degrading enzymes and predict that the whole-cell catalyst will be a viable alternative to protein purification-based approaches for plastic degradation.

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