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Coherent lensless diffraction imaging via multicore fiber for label-efficient microplastics characteristic discrimination

2025
Yunfei Tian, Rongzhou Chen, Yanmin Zhu, Jingyan Chen, Edmund Y. Lam

Summary

Researchers developed a lightweight lensless imaging method using multicore fiber bundles combined with self-supervised machine learning to rapidly discriminate damage states of microplastics, enabling in situ assessment of microplastic deformation status that is difficult to achieve with conventional benchtop microscopy.

The widespread exposure of microplastics (MPs) to environmental and biological systems makes the rapid identification of their damage status a critical task. This deformation influences how MPs migrate, degrade, and interact with organisms, thereby shaping their environmental fate and ecological risk. However, traditional bright-field microscopy suffers from degraded performance under defocused and low-contrast conditions, and benchtop systems are also difficult to deploy in situ in confined spaces. To address these challenges, we propose a lightweight method combining multicore fiber bundle-based coherent defocused imaging with self-supervised representation learning. When only an extremely small number of annotations are provided, semi-supervised constraints, including weighted updates and soft penalties are introduced to enhance cluster-class alignment. This scheme eliminates the need for holographic reconstruction and features a thin, flexible probe, making it suitable for in-situ and online detection in confined environments. The results demonstrate that combining physically enhanced coherent imaging with self-supervised features enables robust differentiation between damaged and undamaged MPs at low annotation cost, and provides a feasible path for on-site deployment.

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