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A genetic tool to express long fungal biosynthetic genes

Fungal Biology and Biotechnology 2023 10 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 40 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Leo Kirchgaessner, Leo Kirchgaessner, Jacob M. Wurlitzer, Jacob M. Wurlitzer, Paula Sophie Seibold, Paula Sophie Seibold, Malik Rakhmanov, Markus Gressler

Summary

Researchers developed a genetic tool that can assemble and express very large fungal genes — over 20,000 base pairs — in a single step within a common laboratory fungus. This system unlocks access to previously inaccessible natural compounds from fungi that can't be cultured in a lab, potentially opening new avenues for discovering bioactive molecules.

The system allows the assembly, targeted integration and expression of genes of > 20 kb size in A. niger in one single step. The system is suitable for evolutionary distantly related SM genes from both Basidiomycota and EDF. This uncovers new SM resources including genetically intractable or non-culturable fungi.

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