On the Novel Process of Pristine Microplastic Bio-fragmentation by Zebrafish (Danio rerio)

Microplastics are highly persistent particles that deliberately contaminate our ecosystem. These small-sized particles can pass through filtering systems into the water bodies, affecting various forms of aquatic and terrestrial life. However, little is known about their fragmentation process within the organism's body. In previous studies, commercially available microplastics were used that are rarely found in the environment naturally, hence they cannot mimic the effects on our surroundings. Therefore, using the zebrafish, Danio rerio we have evaluated the process of bio-fragmentation of ingested pristine polyethene microplastics which are widely used in our daily life. We have also examined their faecal pellets through Field Emission Scanning Electron Microscopy (FE-SEM) and Fourier transform infrared spectroscopy (FTIR). Our results show that zebrafish can potentially bio-fragment the pristine microplastic particles into nano-plastic within a short period of 24 h. Additionally, zebrafish cannot recognize the pristine microplastic particles and can ingest them as food. No mortality occurred during the experiment. Thus, we have identified a natural pathway of microplastic bio-fragmentation, introducing an emerging role of zebrafish in biogeochemical cycling and the fate of plastics.