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Isolation and characterization of microplastics from skin care products; interactions with albumin proteins and in-vivo toxicity studies on Artemia salina
Summary
Researchers isolated and characterized microplastics from two skincare products (face wash and face scrub) and studied their interactions with blood proteins. The study found that ground microplastic particles interacted with bovine and human serum albumin, and toxicity testing on Artemia salina showed adverse effects, suggesting that microplastics from personal care products may pose biological risks.
Skincare products are a significant source of primary microplastics (MPs). This study isolates and characterizes microplastic from two skin care products: face wash (FW-MPs) and face scrub (FS-MPs). Microplastics extracted were around 660 µm in size. The extracted MPs, designated as unground MPs (UG-MPs), were smooth surface and spherical. Ground ones were denoted as the ground MPs (G-MPs) that varied in size and surface shape. G-MPs interacted with bovine serum albumin (BSA) and human serum albumin (HSA). BSA adsorption on FW-MPs was 29%, whereas HSA adsorption was 47%. Contrarily, FS-MPs displayed 17% and 31%. Fluorescence spectroscopy and FE-SEM images showed HSA adsorption on G-MPs was greater than BSA. G-MP interaction changed the life cycle of Artemia salina. UV-Vis and fluorescence spectroscopy were used to study protein adsorption and desorption on G-MPs. A. salina treated to 2.5 mg/mL G-MPs delayed hatching and development and internalized microplastics in the gut at 144 h exposure.
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