Characterization of plastic degrading bacteria isolated from sewage wastewater

Plastic is a fundamental polymer used in routine life and disposed of in sewage. It leads to microplastic pollution in aquatic organisms, introducing it into the food chain and affecting human health. In the present study, samples were collected from sewage wastewater to isolate the bacteria that could potentially reduce plastic. The six samples were incubated with plastic pieces in minimal salt media for 120 days. After 120 days, the weight loss experiment showed that samples SH5B and SH6B degraded 25% plastic. After chemical and molecular characterization, these strains were identified as <i>Pseudomonas</i> sp. SH5B and <i>Pseudomonas aeruginosa</i> SH6B. The Fourier-transform infrared spectroscopy (FTIR) analysis showed peaks shifting, indicating bond stretching, bond bending, and new bond formation. The Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed various new compounds produced during plastic degradation by these bacterial strains. The plastic biodegradation potential makes these bacteria an impending foundation for green chemistry to eradicate tough pollutants from the environment.