Back to papers
0
Article ? AI-assigned paper type based on the abstract. Classification may not be perfect — flag errors using the feedback button. Tier 2 ? Original research — experimental, observational, or case-control study. Direct primary evidence. Detection Methods Human Health Effects Marine & Wildlife Policy & Risk Remediation Sign in to save

Toxicity and Biotransformation of Carbon-based Nanomaterials in Marine Microalgae <em>Heterosigma akashiwo</em>

Preprints.org 2023 5 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Konstantin Pikula, Konstantin Pikula, Konstantin Pikula, Konstantin Pikula, Seyed Ali Johari, Seyed Ali Johari, Seyed Ali Johari, Seyed Ali Johari, Ralph Santos‐Oliveira Ralph Santos‐Oliveira Kirill S. Golokhvast, Seyed Ali Johari, Ralph Santos‐Oliveira Kirill S. Golokhvast, Kirill S. Golokhvast, Kirill S. Golokhvast, Ralph Santos‐Oliveira Kirill S. Golokhvast, Seyed Ali Johari, Ralph Santos‐Oliveira Kirill S. Golokhvast, Ralph Santos‐Oliveira

Summary

Carbon-based nanomaterials including carbon nanotubes, fullerene, graphene, and graphene oxide were tested for toxicity in marine microalgae Heterosigma akashiwo, with carbon nanotubes showing the highest toxicity (EC50 of 18.98 mg/L) primarily through oxidative stress and membrane depolarization.

This work is related to the environmental toxicology risk assessment and evaluation of possible transformation of carbon-based nanomaterials (CNMs) after the contact with marine microalgae. The materials used in the study represent common and widely applied multiwalled carbon nanotubes (CNTs), fullerene (C60), graphene (Gr), and graphene oxide (GrO). The toxicity was evaluated as growth rate inhibition, esterase activity, membrane potential, and reactive oxygen species generation changes. The measurement was performed with flow cytometry after 3, 24, 96 h, and 7 days. Biotransformation of nanomaterials were evaluated after 7 days of microalgae cultivation with CNMs by FTIR and Raman spectroscopy. The calculated toxic level (EC50 in mg/L, 96 h) of ued CNMs reduced in the following order: CNTs (18.98) > GrO (76.77) > Gr (159.40) > C60 (414.0). Oxidative stress and membrane depolarization was the main toxic action of CNTs and GrO. At the same time, Gr and C60 decreased the toxic action with time and had no negative impact on microalgae even at concentration of 125 mg/L. Moreover, C60 and Gr after seven days of the contact with microalgae cells obtained structural deformations.

Read via DOI Download PDF

Sign in to start a discussion.

Share this paper

Post Share Share Pin Email