We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
An In Vivo Whole-Transcriptomic Approach to Assess Developmental and Reproductive Impairments Caused by Flumequine in Daphnia magna
Summary
Researchers used whole-transcriptomic analysis to assess how the antibiotic flumequine causes developmental and reproductive impairments in Daphnia magna, identifying disrupted pathways related to molting, reproduction, and oxidative stress response.
Among veterinary antibiotics, flumequine (FLU) is still widely used in aquaculture due to its efficacy and cost-effectiveness. Although it was synthesized more than 50 years ago, a complete toxicological framework of possible side effects on non-target species is still far from being achieved. The aim of this research was to investigate the FLU molecular mechanisms in Daphnia magna, a planktonic crustacean recognized as a model species for ecotoxicological studies. Two different FLU concentrations (2.0 mg L-1 and 0.2 mg L-1) were assayed in general accordance with OECD Guideline 211, with some proper adaptations. Exposure to FLU (2.0 mg L-1) caused alteration of phenotypic traits, with a significant reduction in survival rate, body growth, and reproduction. The lower concentration (0.2 mg L-1) did not affect phenotypic traits but modulated gene expression, an effect which was even more evident under the higher exposure level. Indeed, in daphnids exposed to 2.0 mg L-1 FLU, several genes related with growth, development, structural components, and antioxidant response were significantly modulated. To the best of our knowledge, this is the first work showing the impact of FLU on the transcriptome of D. magna.
Sign in to start a discussion.
More Papers Like This
Ecological risks of combination of multiple pollutants at environmentally relevant concentrations: Insights from the changes in life history traits, gut microbiota, and transcriptomic responses in Daphnia magna
Researchers exposed Daphnia magna to a combination of 11 pollutants including microplastics, antibiotics, and heavy metals at environmentally relevant ng/L–μg/L concentrations and found significant reductions in heart rate, reproduction, and lifespan, plus gut microbiota and transcriptomic changes — effects that single-pollutant studies would not predict.
Integrated Genomic and Bioinformatics Approaches to Identify Molecular Links between Endocrine Disruptors and Adverse Outcomes
This review examines how whole transcriptome sequencing and genomics technologies can identify molecular links between endocrine-disrupting chemical exposures and adverse outcomes in aquatic organisms, terrestrial animals, and humans.
Machine Learning Models for Identification and Prediction of Toxic Organic Compounds Using Daphnia magna Transcriptomic Profiles
Researchers used transcriptomic profiles from Daphnia magna to train machine learning models capable of identifying and predicting toxicity from 22 organic pollutants in aquatic environments. The models outperformed conventional toxicity tests by providing mechanistic insights into how specific contaminants affect gene expression pathways.
Multi-omics characterisation of Daphnia magna exposed to PFAS and microplastics: transcriptome and gut microbiome datasets
Researchers generated a multi-omics dataset from Daphnia magna exposed to environmentally relevant concentrations of PFOS, PFOA, and PET microplastics, integrating gut microbiome 16S rRNA profiling and whole-organism transcriptomes to enable systems-level investigation of host-microbiome interactions under complex contaminant stress.
Procaine penicillin alters swimming behaviour and physiological parameters of Daphnia magna
Researchers found that procaine penicillin — a beta-lactam antibiotic detected in surface waters — altered swimming behavior, oxygen consumption, heart rate, and thoracic limb movement in the freshwater invertebrate Daphnia magna at concentrations of 11.79 mg/L.