We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Transcriptome analysis of response mechanism to Microcystin-LR and microplastics stress in Asian clam (Corbicula fluminea)
Summary
Researchers used RNA sequencing to analyze gene expression in Asian clam (Corbicula fluminea) hepatopancreas tissue exposed to microcystin-LR, microplastics, and their combination, finding that co-exposure triggered distinct transcriptomic responses compared to individual stressors, with evidence of oxidative and immune pathway disruption.
In this study, we analyzed the hepatopancreas tissues of Asian Clam (Corbicula fluminea) exposed to three different adverse environmental conditions from the same batch using RNA-seq. The four treatment groups included the Asian Clam group treated with Microcystin-LR (MC), the Microplastics-treated group (MP), the Microcystin-LR and Microplastics-treated group (MP-MC), and the Control group. Our Gene Ontology analysis revealed 19,173 enriched genes, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis identified 345 related pathways. The KEGG pathway analysis demonstrated that the MC vs control group and the MP vs control group were significantly enriched in immune and catabolic pathways such as Antigen processing and presentation, Rheumatoid arthritis, Lysosome pathway, Phagosome pathway, and Autophagy pathway. We also evaluated the effects of Microplastics and Microcystin-LR on the activities of eight antioxidant enzymes and immune enzymes in Asian clams. Our study enriched the genetic resources of Asian clams and provided valuable information for understanding the response mechanism of Asian clams to microplastics and microcystin in the environment, through the identification of differentially expressed genes and related pathway analyses from the large number of transcriptome sequences obtained.