Development of automated microplastic identification workflow for Raman micro-imaging and evaluation of the uncertainties during micro-imaging

In this study, an automated identification workflow for Raman micro-imaging (RMI) was developed, and the performance was evaluated by artificial samples of microplastic (MP) microsphere with different sizes and types. Theoretical detection rate and estimated particle size were derived and compared with experimental data. Results show that the proposed workflow can identify plastic types and estimate the size of the MP microspheres under different conditions for most cases. However, size of laser spot and discrepancy between sample surface and focal plane can influence RMI results in two ways. Firstly, small particles are more likely to be detected. Secondly, estimated sizes of particles are more likely to be overestimated. The derived uncertainties can serve as a reference for future experimental design and further investigation of more complex situations. The workflow is accessible online, and interested researchers can adjust the parameter values as necessary to suit their specific circumstances.