An analytical approach to confidence interval estimation of river microplastic sampling

Microplastics (MPs), plastic particles <5 mm in diameter, are emerging ubiquitous pollutants in natural environments, including freshwater ecosystems. As rivers facilitate efficient transport among landscapes, monitoring is crucial for elucidating the origin, dynamics, and fate of MPs. However, standardized methodologies for in situ sampling in freshwater environments remain undefined to date. Specifically, evaluating the sampling error of MP concentration estimates is crucial for comparing results among studies. This study proposes a novel method for computing confidence intervals (CIs) from a single estimate of numerical concentration (expressed in particles·m^-3). MPs are expected to disperse according to purely random processes, such as turbulent diffusion, and to consequently exhibit a random distribution pattern that follows a Poisson point process. Accordingly, the present study introduced a framework based on the Poisson point process to compute CIs, which were validated using MP samples from two urban rivers in Chiba, Japan, obtained using a mesh with an opening size of 335 μm. Random number simulations revealed that the CIs were applicable when ≥10 MPs were present in a sample. Further, when ≥50 MPs were present in a sample, the sampling error (95% CI) was within ±30% of the concentration estimates. The proposed framework allows for the intercomparison of single river MP samples despite the lack of sample replicates. Further, the present study emphasizes that the volume of sampled river water is the only controllable parameter that can reduce the sampling error.