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A review on constructive classification framework of research trends in analytical instrumentation for secondary micro(nano)plastics: What is new and what needs next?
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Surveying over 800 articles from 2004-2022, this review found that Field Flow Fractionation and Hydrodynamic Chromatography are the newest sampling techniques for microplastics, while Fluorescent Molecular Rotors and TD-PTR-MS represent advances in identification. Existing analytical methods remain largely inadequate for nanoplastics, which require urgent methodological development.
Secondary micro(nano)plastics generated from the degradation of plastics pose a major threat to environmental and human health. Amid the growing research on microplastics to date, the detection of secondary micro(nano)plastics is hampered by inadequate analytical instrumentation in terms of accuracy, validation, and repeatability. Given that, the current review provides a critical evaluation of the research trends in instrumental methods developed so far for the qualitative and quantitative determination of micro(nano)plastics with an emphasis on the evolution, new trends, missing links, and future directions. We conducted a meta-analysis of the growing literature surveying over 800 journal articles published from 2004 to 2022 based on the Web of Science database. The significance of this review is associated with the proposed novel classification framework to identify three main research trends, viz. (i) preliminary investigations, (ii) current progression, and (iii) novel advances in sampling, characterization, and quantification targeting both micro- and nano-sized plastics. Field Flow Fractionation (FFF) and Hydrodynamic Chromatography (HDC) were found to be the latest techniques for sampling and extraction of microplastics. Fluorescent Molecular Rotor (FMR) and Thermal Desorption-Proton Transfer Reaction-Mass Spectrometry (TD-PTR-MS) were recognized as the modern developments in the identification and quantification of polymer units in micro(nano)plastics. Powerful imaging techniques, viz. Digital Holographic Imaging (DHI) and Fluorescence Lifetime Imaging Microscopy (FLIM) offered nanoscale analysis of the surface topography of nanoplastics. Machine learning provided fast and less labor-intensive analytical protocols for accurate classification of plastic types in environmental samples. Although the existing analytical methods are justifiable merely for microplastics, they are not fully standardized for nanoplastics. Future research needs to be more inclined towards secondary nanoplastics for their effective and selective analysis targeting a broad range of environmental and biological matrices.
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