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Efficient Biorefinery Based on Designed Lignocellulosic Substrate for Lactic Acid Production

Fermentation 2023 3 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 35 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Ying Wang, Ming Gao

Summary

Researchers developed a simultaneous saccharification and fermentation (SSF) process using Celluclast 1.5L enzyme to produce L-lactic acid from brewer's spent grain, achieving 53.1 g/L optically pure product with a volumetric productivity of 3.65 g/L/h without nutrient supplementation. The approach overcomes glucose-induced carbon catabolite repression by co-utilizing pentose and hexose sugars from lignocellulosic biomass.

The current study investigated the feasibility of developing and adopting a few state-of-the-art fermentation techniques to maximize the efficiency of the lignocellulosic waste bioconversion. There have been various efforts towards utilizing the fermentable sugars released from the specific parts of lignocellulose, i.e., cellulose and hemicellulose. However, complete utilization of carbon sources derived from lignocellulosic biomass remains challenging owing to the generated glucose in the presence of β-glucosidase, which is known as glucose-induced carbon catabolite repression (CCR). To overcome this obstacle, a novel simultaneous saccharification and fermentation (SSF) of lactic acid was designed by using Celluclast 1.5L as a hydrolytic enzyme to optimize the generation and utilization of pentose and hexose. Under the optimal enzyme loading and pH condition, 53.1 g/L optically pure L-lactic acid with a maximum volumetric productivity of 3.65 g/L/h was achieved during the SSF from the brewer’s spent grain without any nutrient supplementation. This study demonstrated the potential of lactic acid production from the designed lignocellulosic substrate.

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