We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Anti-Biofilm Activity of Chlorogenic Acid against Pseudomonas Using Quorum Sensing System
Summary
Researchers investigated the antibiofilm activity of chlorogenic acid against Pseudomonas in drinking water, finding it inhibited biofilm formation in a dose-dependent manner and altered biofilm structure and metabolite composition. Chlorogenic acid shows potential as a natural compound for controlling waterborne bacterial biofilms.
Chlorogenic acid is a secondary metabolite produced by many traditional Chinese medicines. Its physiological activities (antibacterial, anti-inflammatory, antioxidant activities, etc.) have been well described. This study aimed to investigate the effects of chlorogenic acid on the biofilm of drinking water bacteria. The effects of chlorogenic acid on the metabolites of the biofilms were also evaluated. Chlorogenic acid was found to have an anti-biofilm effect against Pseudomonas, resulting in biofilm formation in a dose-dependent manner (0.53-25.4 mM CGA). Moreover, the biofilm structure was visibly attenuated. Furthermore, we identified and characterized 23 differential metabolites and associated two metabolic pathways involving beta-alanine metabolism and pyrimidine metabolism that were altered mostly during biofilm formation. A quantitative real-time PCR assay revealed that chlorogenic acid interfered with the signaling molecule synthesis and transcription regulators using the Las, Pqs and Rhl systems. These findings suggest that chlorogenic acid can be a quorum sensing (QS) inhibitor and inhibit biofilm formation. It may be a promising natural product for the prevention of contaminated drinking water.
Sign in to start a discussion.
More Papers Like This
Metagenomic insights into taxonomic, functional diversity and inhibitors of microbial biofilms
This review examines the structure, diversity, and quorum-sensing mechanisms of microbial biofilms, highlighting metagenomic approaches for understanding how biofilms promote antibiotic resistance and pathogen spread in hospitals and industrial settings.
Biofilm matrix cloaks bacterial quorum sensing chemoattractants from predator detection
Researchers found that bacterial biofilm matrix physically sequesters quorum sensing chemoattractant molecules, preventing detection by predatory bacterivores and providing the biofilm community with a chemical camouflage against grazing pressure.
The application of different methods for indirect microbial development assessment in pilot scale drinking water biofilters
Researchers evaluated methods for monitoring biofilm growth in biological activated carbon filters used in drinking water treatment. Different indirect measurement approaches varied in accuracy and practicality. Reliable biofilm monitoring is important for maintaining filter performance and ensuring treated water is free from microbial and chemical contaminants.
Current methods for monitoring Pseudomonas syringae biofilm development
This paper is not about microplastics — it reviews microscopy and spectroscopy techniques used to study biofilm formation, with a focus on the plant pathogen Pseudomonas syringae.
Phylogenetic Constitution and Survival of Microbial Biofilms Formed on the Surface of Polyethylene Composites Protected with Polyguanidine Biocides
Researchers fabricated polyethylene composites containing immobilized polyguanidine biocides and tested their effectiveness against multispecies microbial biofilms reconstructed from environmental isolates. Polyguanidine biocide suppressed binary and multispecies biofilm growth with a cumulative effect over time, disrupting dense three-dimensional biofilm structures primarily during later formation stages, though cooperative interactions within binary biofilms reduced biocide effectiveness.