We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
[Screening of quality markers and activity verification of Glycyrrhizae Radix et Rhizoma based on small molecule compound-protein interaction].
Summary
Researchers screened 27 chemical compounds in licorice root (Glycyrrhizae Radix) to identify quality markers by measuring how strongly each compound binds to mouse organ proteins. Seven active components were identified, and their anti-inflammatory and antioxidant properties were confirmed in a cell model where macrophages were stressed using microplastics.
In order to solve the problem of weak correlation between quality control components and efficacy of Glycyrrhizae Radix et Rhizoma, this study detected the interaction between small molecular chemical components of Glycyrrhizae Radix et Rhizoma and total proteins of various organs of mice by fluorescence quenching method to screen potential active components. The 27 chemical components in Glycyrrhizae Radix et Rhizoma were detected by HPLC and their deletion rates in 34 batches of Glycyrrhizae Radix et Rhizoma were calculated. Combined with the principle of component effectiveness and measurability, the potential quality markers(Q-markers) of Glycyrrhizae Radix et Rhizoma were screened. RAW264.7 macrophage injury model was induced by microplastics. The cell viability and nitric oxide content were detected by CCK-8 and Griess methods. The levels of inflammatory factors(TNF-α, IL-1β, IL-6, CRP) and oxidative stress markers(SOD, MDA, GSH) were detected by the ELISA method to verify the activity of Q-markers. It was found that the interaction strength between different chemical components and organ proteins in Glycyrrhizae Radix et Rhizoma was different, reflecting different organ selectivity and 18 active components were screened out. Combined with the signal-to-noise ratio of the HPLC chromatographic peaks and between-run stability of the components, seven chemical components such as liquiritin apioside, liquiritin, isoliquiritin apioside, isoliquiritin, liquiritigenin, isoliquiritigenin and ammonium glycyrrhizinate were finally screened as potential Q-markers of Glycyrrhizae Radix et Rhizoma. In vitro experiments showed that Q-markers of Glycyrrhizae Radix et Rhizoma could dose-dependently alleviate RAW264.7 cell damage induced by microplastics, inhibit the secretion of inflammatory factors, and reduce oxidative stress. Under the same total dose, the combination of various chemical components could synergistically enhance anti-inflammatory and antioxidant effects compared with the single use. This study identified Q-markers related to the anti-inflammatory and antioxidant effects of Glycyrrhizae Radix et Rhizoma, which can provide a reference for improving the quality control standards of Glycyrrhizae Radix et Rhizoma.
Sign in to start a discussion.
More Papers Like This
Active compounds of licorice ameliorate microplastics-induced intestinal damage by targeting FADD
Researchers tested whether active compounds from licorice root could protect intestinal cells from damage caused by microplastic exposure in mice, finding that licorice compounds reduced inflammation and oxidative stress in the gut and partially restored intestinal barrier integrity.
Glycyrrhizin Alleviates the Damage Caused by Zearalenone and Protects the Glandular Stomach of Chickens
Researchers investigated whether glycyrrhizin, a natural compound from licorice root, could protect chicken stomach tissue from damage caused by the fungal toxin zearalenone. They found that glycyrrhizin reduced inflammation, oxidative stress, and cell death in glandular stomach cells exposed to the toxin. The study suggests that natural plant extracts may offer protective benefits against common agricultural contaminants in poultry.
Table1_Identifying of Anti-Thrombin Active Components From Curcumae Rhizoma by Affinity-Ultrafiltration Coupled With UPLC-Q-Exactive Orbitrap/MS.DOCX
This supplementary data file accompanies a study screening herbal extracts from Curcuma for compounds that inhibit the clotting enzyme thrombin, using liquid chromatography-mass spectrometry. The research identifies natural plant compounds with potential anticoagulant properties from traditional medicinal plants.
Anti-rheumatoid arthritis potential of Acorus calamus L. extract by interleukin-17 inhibition: Molecular insights through an in silico study
This paper is not about microplastics. It is a pharmacology study that investigated natural compounds from Acorus calamus plant extract as potential treatments for rheumatoid arthritis by targeting the inflammatory protein interleukin-17. The study uses molecular docking simulations and drug analysis with no connection to microplastic pollution or health effects.
The potential mechanism of huazhuojiedu decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation
Researchers used network pharmacology to identify 119 active components and 146 potential targets of the traditional Chinese medicine formula Huazhuojiedu decoction relevant to ulcerative colitis, then validated key predicted mechanisms in dextran sulfate sodium-induced rat colitis models, elucidating multi-target pathways underlying the formula's clinically observed therapeutic effects.