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Article Tier 2 Detection Methods Marine & Wildlife Policy & Risk Remediation Sign in to save

A rapid and portable fluorescence spectroscopy staining method for the detection of plastic microfibers in water

The Science of The Total Environment 2023 14 citations

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A fluorescent dye (PTSA) that selectively binds to synthetic polymers was developed into a portable, low-cost method for detecting microfibers in fresh, estuarine, and seawater samples, validated against FTIR and fluorescence microscopy. The technique could help researchers and regulators monitor microplastic fiber pollution quickly and affordably in the field, especially in under-resourced settings.

Study Type Environmental

We propose a simple technique for microplastic detection based on their interaction with a hydrophilic and anionic fluorescent dye 1,3,6,8 pyrene tetrasulfonate (PTSA). Synthetic microfibers derived from surgical face masks (an abundantly generated plastic waste post COVID) were considered as model microplastics. The interactions between microfibers and the dye were studied as a function of physiological parameters (pH, contact time and temperature), external agents, dye dosage and polymer variants. A pocket-sized photometer (by Lovibond Tintometer group) was employed for the detection and further validated using advanced equipment set-ups (fluorescence microscope, Fourier transform infrared spectroscopy and benchtop spectrofluorometer). Risk assessment studies were conducted on Artemia salina as a model organism. As a risk mitigation strategy, dye recovery followed by sunlight mediated degradation were performed. The detection study was performed in real water samples collected from fresh, estuarine and seawater samples spiked with microfibers. As an outcome, an optimized standard operational conditions were determined for the effective detection of synthetic microfibers. The data obtained could have scientific and industrial impact, in particular for experts working in the broad arena of clean water, who are specifically interested in developing cost-effective solutions for effective detection and biomonitoring of emerging pollutants.

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