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Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions
Summary
This study isolated and characterized bacteria from hydrocarbon-contaminated soil that can degrade kerosene, with potential applications in bioremediation of fuel-contaminated sites. Research on microbial hydrocarbon degradation contributes to the broader field of biodegradation of synthetic organic pollutants including plastics.
Bacteria capable of decomposing hydrocarbon rocket power kerosene T-1 were isolated and studied.In the course of the studies, 30 isolates of microbial cultures were isolated from soils contaminated with hydrocarbon rocket fuel, of which 9 isolates were selected that actively assimilate kerosene T-1 as the only headwater of carbon.The strains that showed the best results in these screening analyses on four nutrient media with a concentration of T-1 kerosene 1% (10 g/kg) grow well 9 isolates of culture microorganisms: 4, 8, 14, 23, 5, 18, 20, 25 and yeast 12/5.Isolates on a medium with a concentration of T-1 kerosene 2% (20 g/kg) and 5% (50 g/kg) showed good growth in bacterial cultures 5, 18, 20, 25, and yeast 12/5.The selected microorganisms were identified by physiological and biochemical characteristics: 5 -Arthrobacter sp., 18 -Acinetobacter calcoaceticum, 20 -Bacillus sp., 25-Micrococcus roseus, 12/5-Candida sp.Cultivation conditions for isolated microorganisms were created.It has been established that the optimal development temperature for the culture of Arthrobacter sp. 5 is 25-30°C, Acinetobacter calcoaceticum.18 is 30-35 ° , Micrococcus roseus.25 is 25-37 ° .The optimal duration of cultivation of Candida sp.12/5 is 1 day, for the rest of the studied cultures -2 days.
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