Microplastisphere antibiotic resistance genes: A bird's-eye view on the plastic-specific diversity and enrichment

The microplastisphere is a dense consortium of metabolically active microorganisms that develops on the surface of microplastics. Since the discovery that it harbors antibiotic resistance genes (ARGs), there has been a quest to decipher the relationship between ARG occurrences and selective enrichment with plastic types, which is important to understand their fate in diverse environmental settings. Nonetheless, it remains a neglected topic, and this developing field of microplastics research could benefit from a comprehensive review to acquire a deeper understanding of the most recent advances and drive scientific progress. Accordingly, the goal of this review is to critically discuss and provide an in-depth assessment of the evidence of ARGs' global nature in microplastispheres, as well as explore factors that influence them directly and indirectly, highlighting important concerns and knowledge gaps throughout the article. By comprehensively covering them, we underscore the potential environmental implications associated with microplastisphere ARGs. From our analysis, it emerged that microplastisphere ARGs are likely to be impacted not only by differences in microplastic types and characteristics but also by how their environments are shaped by other agents such as physiochemical properties, socioeconomic factors, and contaminants coexistence, influencing ARG subtype, incidence, abundance, and selective enrichment. The intricate relationship of microplastisphere ARGs to environmental conditions and plastic types calls for multilevel investigations to clearly assess the environmental fate of microplastics. We anticipate that this review could assist researchers in strengthening their foundation and identifying efforts to advance knowledge in this research field.