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Optimization of an analytical method for the characterization of microplastics in clams using Raman microspectroscopy
Summary
Researchers developed and optimized an analytical method for extracting and characterizing microplastics from the clam Chamelea gallina using Raman microspectroscopy, selecting appropriate filters and evaluating recovery rates to maximize reliability. The method was applied to clam samples collected from the Adriatic Sea coast across the Marche, Abruzzo, and Molise regions of Italy to conduct a preliminary assessment of microplastic contamination levels.
The first two activities of the PhD thesis project are described hereunder. Firstly, the sampling of clam Chamelea gallina was planned in order to obtain a representative number of samples and carried out in the coasts of the Adriatic Sea included in the Marche, Abruzzo and Molise regions. Secondly, a method for the extraction from clams and the chemical characterization of microplastics (MPs) with Raman microspectroscopy (μRaman) was developed, which took into account the type of filters and the recoveries obtained. The optimized method was applied for the analysis of the samples, for a preliminary assessment of contamination levels.