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International Society for Extracellular Vesicles workshop. QuantitatEVs: Multiscale analyses, from bulk to single extracellular vesicle

Journal of Extracellular Biology 2024 5 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 55 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Manuela Basso, Anna Kashkanova, Alessandro Gori, Caterina Nardella, Marco P. Monopoli, Martin Blessing, Mari Palviainen, Marco P. Monopoli, Marija Holcar, Ioannis Sotiropoulos, Paolo Bergese, Sylwia Bobis‐Wozowicz, Paolo Bergese, Vito D’Agostino, Paolo Bergese, Paolo Bergese, Elena Casarotto, Yari Ciani, Shiro Suetsugu, Alice Gualerzi, Lorena Martín‐Jaular, Daniela Boselli, Marco P. Monopoli, Anna Kashkanova, Pietro Parisse, Lien Lippens, Martina Pagliuca, Martin Blessing, Roberto Frigerio, Thibaut Fourniols, Thibaut Fourniols, Ana Meliciano, A. Fietta, Paolo Vincenzo Fioretti, Paolo Vincenzo Fioretti, Karolina Soroczyńska, Silvia Picciolini, Amanda Salviano‐Silva, Paolo Bergese, Davide Zocco, Davide Zocco, Marcella Chiari, Guido Jenster, Kenneth W. Witwer, Levi Waldron, Aleksandar Milosavljevic, John P. Nolan, Paolo Bergese, Marco P. Monopoli, Kenneth W. Witwer, Benedetta Bussolati, Dolores Di Vizio, Juan Falcon Perez, Juan Falcon Perez, Metka Lenassi, Marina Cretich, Francesca Demichelis

Summary

This workshop report summarizes discussions among scientists about improving methods for measuring and analyzing extracellular vesicles, which are tiny particles released by cells. Researchers explored strategies for standardizing both laboratory techniques and computational approaches, from bulk samples down to individual vesicles. The report identifies key challenges and emerging technologies needed to advance this rapidly growing field of biomedical research.

The 'QuantitatEVs: multiscale analyses, from bulk to single vesicle' workshop aimed to discuss quantitative strategies and harmonized wet and computational approaches toward the comprehensive analysis of extracellular vesicles (EVs) from bulk to single vesicle analyses with a special focus on emerging technologies. The workshop covered the key issues in the quantitative analysis of different EV-associated molecular components and EV biophysical features, which are considered the core of EV-associated biomarker discovery and validation for their clinical translation. The in-person-only workshop was held in Trento, Italy, from January 31<sup>st</sup> to February 2<sup>nd</sup>, 2023, and continued in Milan on February 3<sup>rd</sup> with "Next Generation EVs", a satellite event dedicated to early career researchers (ECR). This report summarizes the main topics and outcomes of the workshop.

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