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Quality of Sperm Simmental Bulls and Success of Artificial Insemination with the Addition of Nanocalcium Phosphate in Tris Aminomethane Egg Yolk Diluent Using Semen Storage Ampoules from Nanocalcium Silicophosphate Biomaterials
Summary
This study found that adding 1% nanocalcium phosphate to semen preservation diluent and using nanocalcium silicophosphate biomaterial ampoules improved Simmental bull sperm quality during cryopreservation. The enhanced storage approach maintained higher sperm motility and viability over four days, potentially improving artificial insemination success rates in livestock breeding.
Success in Artificial Insemination (AI) is largely determined by the quality of the stored semen, therefore storage devices, especially straws or ampoules, are an important part that cannot be separated from the overall semen cryopreservation technology. Making a prototype of semen storage ampoules from nanocalcium silicophosphate biomaterials is based on the need for straws or ampoules. Semen storage in spermatozoa cryopreservation technology for the needs of AI and livestock breeding and from the results of an examination of previously patented inventions. The aim of this research was to determine the quality of simental cow sperm and the success of AI with the addition of nanocalcium phosphate to the Tris aminomethane egg yolk diluent using semen storage ampoules made from nanocalcium silicophosphate biomaterial. This research method uses laboratory research with a factorial Completely Randomized Design (CRD) consisting of 2 factors, namely the diluent factor consisting of 2 treatments (tris aminomethane egg yolk and tris aminomethane egg yolk + phosphate 1% w/v) and the shelf life factor. Consisting of 5 treatments (0, 1, 2, 3, 4 days). The second factor is the use of ampoules and without ampoules. Each treatment was repeated 10 times. The results of the research showed that the addition of nanocalcium phosphate to the egg yolk tris aminomethane diluent using semen storage ampoules made from nanocalcium silicophosphate biomaterial had a very significant effect (p<0.01) on motility, viability and abnormalities. Likewise, the AI results show the NRR, CR, and S/C values in the good category. This study concluded that the addition of phosphate to the egg yolk tris aminomethane diluent can increase the durability of semen for up to three days at room temperature and with storage at 5°C for up to 3-5 days, likewise, the results of artificial insemination success tests are also good and appropriate standard.
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