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At second glance: The importance of strict quality control – A case study on microplastic in the Southern Ocean key species Antarctic krill, Euphausia superba
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Researchers analyzed krill stomach contents from the Southern Ocean for microplastic contamination using hyperspectral imaging FTIR spectroscopy, applying strict quality controls across different sample volumes and extraction approaches. The study emphasized that rigorous quality control is essential for producing reliable microplastic detection results in small biological samples.
The stomach content of 60 krill specimens from the Southern Ocean were analyzed for the presence of microplastic (MP), by testing different sample volumes, extraction approaches, and applying hyperspectral imaging Fourier-transform infrared spectroscopy (μFTIR). Strict quality control was applied on the generated results. A high load of residual materials in pooled samples hampered the analysis and avoided a reliable determination of putative MP particles. Individual krill stomachs displayed reliable results, however, only after re-treating the samples with hydrogen peroxide. Before this treatment, lipid rich residues of krill resulted in false assignments of polymer categories and hence, false high MP particle numbers. Finally, MP was identified in 4 stomachs out of 60, with only one MP particle per stomach. Our study highlights the importance of strict quality control to verify results before coming to a final decision on MP contamination in the environment to aid the establishment of suitable internationally standardized protocols for sampling and analysis of MP in organisms including their habitats in Southern Ocean and worldwide.
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When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.