Morphological and Chemical Characteristics of Microplastics in Surface Water of the Vaal River, South Africa

Abstract Microplastics research in South Africa has focused on marine and coastal areas, with limited studies reporting microplastics in freshwaters. To address this gap, we conducted this study to investigate the presence, abundance, physical characteristics, and chemical composition of microplastics in the surface water of the Vaal River, one of the most important freshwater systems in South Africa. Twenty-two surface water samples were collected along 60 km of the middle part of the river using a 55 μm plankton net. Samples were treated by acid digestion, and MPs were separated by density separation. Physical identification was performed using a Nikon stereomicroscope, and chemical composition was identified using Raman spectroscopy. MPs were observed in all samples with 0.13 to 2.52 particles/m 3 concentrations. Microplastics ranged in size from 0.06 – 4.95 mm, with more than 89% less than 2 mm, and 81% were fragments (39%) and fibers (42%). Microplastics were observed in numerous colours, of which green, black, and blue were the most dominant, representing 22%, 19%, and 18%, respectively. Chemical analysis revealed the polymer types as high-density polyethylene (HDPE), polypropylene (PP), and low-density polyethylene (LDPE). In addition, two colourants, pigment yellow 83 and carbon black, were detected. This study provides new data on microplastic occurrence and characteristics in the Vaal River system and offers a baseline for future monitoring. Our findings confirm that the Vaal River is under a real threat of microplastic contamination, which could impact its role as a significant freshwater system in South Africa. Highlights • Microplastics were prevalent in the surface water of the Vaal River. • Fibrous, small-sized, and coloured microplastics were most abundant. • Polymers were identified as HDPE, LDPE, and PP.