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Nanoplastics composite norfloxacin induced changes in conformation and function of lysozyme and differential effects of co-exposure contamination

The Science of The Total Environment 2024 6 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 55 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Yuntao Qi, Shuqi Guo, Yuntao Qi, Xiangxiang Li, Hengyu Song, Falin He, Xiangxiang Li, Hengyu Song, Xiangxiang Li, Hengyu Song, Falin He, Xiangxiang Li, Yuntao Qi, Yuntao Qi, Yuntao Qi, Yuntao Qi, Shuqi Guo, Hengyu Song, Shuqi Guo, Xiangxiang Li, Yuntao Qi, Shaoyang Hu, Shuqi Guo, Shaoyang Hu, Shuqi Guo, Jiang Pin, Shuqi Guo, Shuqi Guo, Yuntao Qi, Jiang Pin, Falin He, Shaoyang Hu, Shaoyang Hu, Shuqi Guo, Shaoyang Hu, Shuqi Guo, Shuqi Guo, Shuqi Guo, Shuqi Guo, Shuqi Guo, Yuntao Qi, Yuntao Qi, Yuntao Qi, Houquan Tang, Houquan Tang, Xiangxiang Li, Falin He, Xiangxiang Li, Shaoyang Hu, Rutao Liu Falin He, Yuntao Qi, Houquan Tang, Zaifeng Wang, Zaifeng Wang, Guang Tian, Guang Tian, Yuntao Qi, Xiangxiang Li, Shaoyang Hu, Shaoyang Hu, Guang Tian, Guang Tian, Xiangxiang Li, Rutao Liu Yuntao Qi, Xiangxiang Li, Falin He, Xiangxiang Li, Yuntao Qi, Yuntao Qi, Yuntao Qi, Xiangxiang Li, Yuntao Qi, Shuqi Guo, Rutao Liu Yuntao Qi, Shaoyang Hu, Yuntao Qi, Shuqi Guo, Yuntao Qi, Shuqi Guo, Rutao Liu Rutao Liu Rutao Liu Rutao Liu Xiangxiang Li, Xiangxiang Li, Hengyu Song, Hengyu Song, Hengyu Song, Xuan Ge, Xuan Ge, Yuntao Qi, Yuntao Qi, Yuntao Qi, Shuqi Guo, Hengyu Song, Shuqi Guo, Rutao Liu Shuqi Guo, Guang Tian, Shaoyang Hu, Rutao Liu Guang Tian, Shaoyang Hu, Xiangxiang Li, Rutao Liu Shaoyang Hu, Rutao Liu Rutao Liu Rutao Liu Rutao Liu Falin He, Rutao Liu Shuqi Guo, Shuqi Guo, Rutao Liu Rutao Liu Xiangxiang Li, Rutao Liu Rutao Liu Guang Tian, Falin He, Falin He, Guang Tian, Guang Tian, Guang Tian, Yuntao Qi, Yuntao Qi, Shaoyang Hu, Rutao Liu Yuntao Qi, Rutao Liu Shaoyang Hu, Yuntao Qi, Xiangxiang Li, Xiangxiang Li, Xiangxiang Li, Xiangxiang Li, Guang Tian, Shuqi Guo, Shaoyang Hu, Shuqi Guo, Rutao Liu Xiangxiang Li, Shaoyang Hu, Shuqi Guo, Yuntao Qi, Shuqi Guo, Rutao Liu Yuntao Qi, Guang Tian, Rutao Liu Shaoyang Hu, Guang Tian, Rutao Liu Shaoyang Hu, Shaoyang Hu, Yuntao Qi, Rutao Liu Xuan Ge, Guang Tian, Rutao Liu Yuntao Qi, Rutao Liu Rutao Liu Rutao Liu Rutao Liu Yuntao Qi, Rutao Liu Rutao Liu Yuntao Qi, Shaoyang Hu, Rutao Liu

Summary

Researchers used spectroscopy and molecular docking to show that nanoplastics and the antibiotic norfloxacin form stable co-aggregates that bind near the active site of lysozyme, reducing its enzymatic activity by nearly 40% and altering the protein's secondary structure more severely than either contaminant alone.

The concurrent environmental contamination by nanoplastics (NPs) and norfloxacin (NOR) is a burgeoning concern, with significant accumulations in various ecosystems and potential ingress into the human body via the food chain, posing threats to both public health and ecological balance. Despite the gravity of the situation, studies on the co-exposure contamination effects of these substances are limited. Moreover, the response mechanisms of key functional proteins to these pollutants are yet to be fully elucidated. In this work, we conducted a comprehensive assessment of the interaction mechanisms of NPs and NOR with lysozyme under both single and co-exposure condition, utilizing dynamic light scattering, ζ-potential measurements, multi-spectroscopy methods, enzyme activity assays and molecular docking, to obtain a relationship between the compound effects of NPs and NOR. Our results indicate that NPs adsorb NOR on their surface, forming more stable aggregates. These aggregates influence the conformation, secondary structure (α-Helix ratio decreased by 3.1 %) and amino acid residue microenvironment of lysozyme. And changes in structure affect the activity of lysozyme (reduced by 39.9 %) with the influence of composited pollutants exerting stronger changes. Molecular simulation indicated the key residues Asp 52 for protein function located near the docking site, suggesting pollutants preferentially binds to the active center of lysozyme. Through this study, we have found the effect of increased toxicity on lysozyme under the compounded conditions of NPs and NOR, confirming that the increased molecular toxicity of NPs and NOR is predominantly realized through the increase in particle size and stability of the aggregates under weak interactions, as well as induction of protein structural looseness. This study proposes a molecular perspective on the differential effects and mechanisms of NPs-NOR composite pollution, providing new insights into the assessment of in vitro responses to composite pollutant exposure.

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