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Brief guidelines for zebrafish embryotoxicity tests
Summary
Researchers published step-by-step guidelines for using zebrafish embryos as a model to test the toxicity of environmental pollutants including nanoplastics, heavy metals, and pesticides, covering everything from breeding methods to measuring physical defects in developing embryos. Zebrafish are a widely used research tool because their transparent embryos develop quickly and share many biological similarities with humans.
There has been growing emphasis on environmental pollutants, including heavy metals, pesticides, and nanoplastics, owing to the escalating significance of environmental pollution as a major global issue. Various toxicities induced by these compounds have been consistently reported, and many cell lines and animal models have been used in toxicity studies. Zebrafish are one of the most widely used animal models for verifying the toxic effects of environmental pollutants, owing to their many advantages. In this study, we provide brief guidelines for zebrafish maintenance and mating methods, toxicant treatments, survival measurements, and morphological abnormalities.