Nanoplastic impact on bone microenvironment: A snapshot from murine bone cells.
Zenodo (CERN European Organization for Nuclear Research)2024
Score: 45
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Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Giulia Sauro,
Giulia Sauro,
Giulia Sauro,
Giulia Sauro,
Domenica Giannandrea,
Domenica Giannandrea,
Domenica Giannandrea,
Domenica Giannandrea,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Marco Parolini
Marco Parolini
Marco Parolini
Beatrice De Felice,
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Marco Parolini
Marco Parolini
Beatrice De Felice,
Beatrice De Felice,
Valentina Citro,
Valentina Citro,
Beatrice De Felice,
Marco Parolini
Beatrice De Felice,
Marco Parolini
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Beatrice De Felice,
Marco Parolini
Beatrice De Felice,
Marco Parolini
Marco Parolini
Giulia Sauro,
Marco Parolini
Beatrice De Felice,
Marco Parolini
Giulia Sauro,
Marco Parolini
Giulia Sauro,
Giulia Sauro,
Giulia Sauro,
Marco Parolini
Beatrice De Felice,
Giulia Sauro,
Giulia Sauro,
Giulia Sauro,
Marco Parolini
Beatrice De Felice,
Beatrice De Felice,
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Beatrice De Felice,
Valentina Citro,
Valentina Citro,
Raffaella Chiaramonte,
Raffaella Chiaramonte,
Chiu, Martina,
Nbm Conese,
Nbm Conese,
Natalia Platonova,
Beatrice De Felice,
Marco Parolini
Marco Parolini
Marco Parolini
Natalia Platonova,
Natalia Platonova,
Marco Parolini
Marco Parolini
Marco Parolini
Chiu, Martina,
Natalia Platonova,
Marco Parolini
Natalia Platonova,
Marco Parolini
Beatrice De Felice,
Elena Lesma,
Marco Parolini
Elena Lesma,
Natalia Platonova,
Marco Parolini
Marco Parolini
Casati Lavinia,
Marco Parolini
Raffaella Chiaramonte,
Raffaella Chiaramonte,
Casati Lavinia,
Casati Lavinia,
Casati Lavinia,
Marco Parolini
Nbm Conese,
Marco Parolini
Nbm Conese,
Marco Parolini
Marco Parolini
Chiu, Martina,
Marco Parolini
Chiu, Martina,
Beatrice De Felice,
Casati Lavinia,
Casati Lavinia,
Casati Lavinia,
Beatrice De Felice,
Marco Parolini
Beatrice De Felice,
Casati Lavinia,
Elena Lesma,
Marco Parolini
Marco Parolini
Elena Lesma,
Raffaella Chiaramonte,
Raffaella Chiaramonte,
Marco Parolini
Marco Parolini
Marco Parolini
Marco Parolini
Summary
This study examined how nanoplastics affect bone cell function in a murine model, investigating effects on osteoblasts and osteoclasts that govern bone formation and resorption in the bone microenvironment. Nanoplastic exposure altered bone cell activity, suggesting that daily plastic particle exposure could have long-term implications for bone health.
We live in a plastic world: every day, a wide array of plastics of different compositions, shapes, and sizes contaminate the environment and enter the food chain, profoundly affecting our lives and human health. Nanoplastics (NPs) are emerging as one of our time's most important environmental pollutants to which human organisms are exposed due to their capability to cross cell membranes and induce cellular toxicity. Recent studies reported that NPs toxic effect is partially mediated by oxidative stress (OS). Reactive oxygen species (ROS) are among the most critical factors involved in disrupting skeleton integrity and promoting bone resorption. This study aimed to investigate the impact of OS derived from NP exposure on bone microenvironment. We treated murine bone cells (MC3T3-E1 pre-osteoblast, MLOY-4 osteocyte-like cells, and RAW264.7 pre-osteoclast) with NPs at different concentrations. We found that NPs, once internalized, affect cell viability (assessed by MTT assay), induce ROS production (evaluated by DCFHDA assay), and trigger caspase 3/7 mediated apoptosis (assessed by a fluorometric assay) in all the cell lines tested. NPs hamper the pre-osteoblasts migration capability and potentiate the osteoclastogenesis in pre-osteoclasts by increasing their differentiation toward osteoclasts. We also performed a transcriptomic analysis evaluating gene expression in bone remodeling. Our results showed that OS by NPs induces in RAW264.7 cells an increased expression of genes related to the osteoclastogenic commitment of pre-osteoclasts, together with a disruption of the genes involved in osteoblastogenesis and modulation of inflammatory pathway-related genes in MC3T3-E1 and MLOY-4 cells, respectively. A better understanding of the impact of NPs on bone cell activities resulting in vivo in impaired bone turnover could give more information on the toxicity consequence of NPs on bone mass and the subsequent public health problems, such as bone disease. Also see: https://micro2024.sciencesconf.org/559009/document