Exogenous signaling molecules N-acyl-homoserine lactones promotes the reconstruction of sludge particles after impact with highly concentrated urea-formaldehyde resin microplastics

In this study, exogenous N-acyl-homoserine lactones (AHLs) was added to resist the stress by high concentration (0.5 g/L) of urea formaldehyde resin microplastics (UF-MPs) on anaerobic granular sludge (AnGS), aiming to provide a viable strategy for AnGS to withstand elevated levels of UF-MPs toxicity elucidate the intricate regulatory mechanism of AHL-mediated AnGS-QS regulation. The results showed that the three different signaling molecules (C4-HSL, C6-HSL, and C8-HSL) improved the performance of AnGS under high concentration (0.5 g/L) urea-formaldehyde resin stress, and increased sludge COD removal (4.48%, 4.76%, and 3.35%, respectively) and methanogenic activity (8.38%, 1.92%, and 18.76%, respectively). The addition of C4-HSL has the best effect on sludge particle size and strength, which is attributed to the fact that C4-HSL can significantly increase the content of polysaccharides and proteins in tightly bound extracellular polymeric substances (TB-EPS) (27.1% and 27.1%, respectively). C8-HSL most obviously promotes energy metabolism and EPS biosynthesis gene expression. Metagenomic analysis showed that trace AHLs could promote the abundance of enzymes and functional genes related to the main pathway of methane metabolism, increase the relative abundance of Methanothrix of acetophilic methanogens from 27.79% in the control group to 27.85% (C4-HSL), 28.90% (64-HSL), and 30.03% (C8-HSL), thereby improving community stability.