Chronic effects of irregular and fibril microplastics on Artemia franciscana in a benthic environment: Size and shape-dependent toxicity

Marine ecosystems are contaminated by plastic products, particularly microplastics (MPs), which settle on the seafloor and affect benthic organisms. This study explores the toxicity of irregular fibril-shaped MPs of various sizes and lengths on Artemia franciscana. We exposed juvenile A. franciscana to irregular-shaped MPs of three sizes, small (<20 μm), medium (40-70 μm), and large (>120 μm), and MP fibers of two length categories, short (200-300 μm) and long (3 mm), in a sand-layered benthic system. The concentrations of the MPs were maintained at 0.05-20 mg/L, and the study was conducted over a 28-d of chronic period. Among all the MPs considered in this study, the small and short MPs exerted the most severe effects (causing mortality, growth inhibition, gut damage, alterations in movement, and a decrease in positive phototaxis). Our study highlights the importance of considering the morphological characteristics of MPs for analyzing their toxicity to aquatic eco-receptors.