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Biorremediação do Bisfenol A pela lacase de Pycnoporus sanguineus (L.) Murril após indução por resíduos de casca de Caryocar brasiliense Camb
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This study tested the ability of laccase enzyme from the fungus Pycnoporus sanguineus to bioremediate bisphenol A (BPA) from water, measuring degradation rates and breakdown products. The enzyme achieved significant BPA removal under optimized conditions, offering a biological treatment approach.
Este estudo utilizou a enzima lacase, produzida pelo fungo Pycnoporus sanguineus, para bior-remediação do BPA avaliando a casca de pequi (Caryocar brasiliense) como indutora natural da produção de lacase. O controle negativo foi realizado sem indutor e o positivo na presença de indutor sintético. Para a biorremediação, 510 U de atividade da lacase foi adicionado à 10 mL de solução de BPA à 1 mg/mL sendo mantido sob agitação (25ºC/48h). A porcentagem de remoção foi avaliada por HPLC após os períodos de 0; 2; 4; 6; 8; 10; 12; 24 e 48 horas de rea-ção. A lacase induzida pela casca de pequi se mostrou altamente eficaz, alcançando uma ativi-dade de 3.357,436 U/mL após seis dias. Ademais, a enzima demonstrou estabilidade térmica, com melhor atividade a 30°C e pH 5. A aplicação da lacase à solução de BPA resultou em 100% de remoção do contaminante após 24 horas de reação. Esses resultados indicam que a casca de C. brasiliense induz uma maior produção de lacase contribuindo para um processo de biorremediação eficiente. A utilização da lacase de P. sanguineus no tratamento de águas con-taminadas com BPA oferece uma solução ambiental sustentável, promovendo a saúde humana conforme a abordagem integrada de saúde única.
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