Effects of naturally aged microplastics on arsenic and cadmium accumulation in lettuce: Insights into rhizosphere microecology

Naturally aged microplastics (NAMPs) are commonly found in farmland soils contaminated with heavy metals (HMs), such as arsenic (As) and cadmium (Cd); yet their combined effects on soil-plant ecosystems remain poorly understood. In this study, we investigated the toxic effects of NAMPs and As-Cd on lettuce, considering the influence of earthworm activity, and examined changes in As-Cd bioavailability in the rhizosphere. Four experimental systems were established: soil-only, soil-lettuce, soil-earthworms, and soil-lettuce-earthworms systems, with four NAMPs concentrations (0, 0.1, 0.5, 1 %). Our results showed that exposure to 0.1 % NAMPs reduced As accumulation in lettuce shoots (0.17-0.25 mg kg) and roots (1.13-1.72 mg kg), while increasing biomass and enhancing root growth by alleviating toxicity. In contrast, the combined stress of higher NAMPs concentration (0.5 %/1 %) and As-Cd caused a 28.4-58.4 % reduction in root activity, which stimulated low-molecular-weight organic acid (LMWOA) secretion in the rhizosphere, increasing the bioavailability of As and Cd and enhancing their absorption by lettuce. Partial least squares path modeling (PLS-PM) revealed that co-exposure altered LMWOA content, soil enzyme activity, and microbial community stability in the rhizosphere, ultimately influencing the bioavailability and uptake of As and Cd by lettuce.