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Researchers investigated how multiple global change stressors — including microplastics, salinity, heat, drought, and surfactants — interact in urban soils using a subtractive experimental design, finding that combined effects frequently differ from individual impacts and that mitigating the strongest multi-stressor factors is more effective for restoration than targeting those with significant isolated effects.

The presence of multiple global change factors affects most ecosystems, especially urban soils, which face stressors like heat, drought, road salt, nitrogen deposition, surfactants, microplastics, and heavy metals. These factors are often studied in isolation or in pairs, leaving their combined effects poorly understood. Importantly, understanding how removing a single factor impacts an ecosystem amidst multiple stressors is crucial for effective restoration.We explored this through a subtractive design, comparing single-factor treatments (addition) to treatments where a specific factor was removed (subtraction). The results varied: salinity had predominantly negative effects, warming had positive effects, and drought had mixed effects as single factors. However, removing these factors from a multi-factor context generally improved soil properties and biological processes. Microplastics and surfactants enhanced microbial activity individually but showed no such benefit in multi-factor scenarios.Our findings highlight that the combined effects of factors often differ from their individual impacts. In restoration, priority should be given to mitigating factors with the strongest negative influence in multi-stressor contexts, rather than targeting those with significant isolated effects. This approach can better address the complexities of global change in ecosystems.

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Researchers investigated how multiple global change stressors — including microplastics, salinity, heat, drought, and surfactants — interact in urban soils using a subtractive experimental design, finding that combined effects frequently differ from individual impacts and that mitigating the strongest multi-stressor factors is more effective for restoration than targeting those with significant isolated effects.

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Researchers investigated how multiple global change stressors — including microplastics, salinity, heat, drought, and surfactants — interact in urban soils using a subtractive experimental design, finding that combined effects frequently differ from individual impacts and that mitigating the strongest multi-stressor factors is more effective for restoration than targeting those with significant isolated effects.

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A subtractive experimental design in urban soils found that removing individual global change stressors — including microplastics — from a multi-factor background generally improved soil properties and biological processes, and that the combined effects of stressors often differed from what any single factor would predict alone. This is important for understanding microplastic impacts in real-world soils, where plastic pollution rarely occurs in isolation from other stressors like heat, drought, and chemical contamination.

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An urban soil experiment using a subtractive design (removing individual stressors from a multi-stressor scenario including microplastics, heat, road salt, drought, nitrogen, and surfactants) found that combined stressors produce very different — often worse — effects than any single stressor causes alone. For microplastic research, the key implication is that lab studies testing microplastics in isolation likely underestimate real-world harms, because in urban soils microplastics always co-occur with multiple other stressors.

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Researchers provided R code and associated data examining how multiple global change factors including microplastic contamination affect urban soils, which face compounding stressors from pollution, altered hydrology, and reduced biodiversity, using statistical modelling to disentangle the effects of co-occurring environmental pressures.

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