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Identification of Microplastics in Aquatic Environments Using Oxidative Treatment and Automated Image Analysis

Figshare 2025
Chu, Ruiqi, Zong, William, Ye, Jingyi, Kissiov, Thea, (Andrew) Yu, Zhixing, Kalakota, Veera, Rengaraj, Dakshin, (Martina) Li, Xiwen, Gomez-Godinez, Veronica, Shi, Linda Z.

Summary

Researchers developed a cost-effective and replicable method for detecting microplastics in freshwater environments using oxidative treatment to digest organic matter from water samples, enabling cleaner isolation and more accurate identification of MP particles without requiring expensive instrumentation.

Study Type Environmental

This study presents a cost-effective and replicable method for detecting microplastics in freshwater environments. Water samples from six Southern California lakes and creeks were filtered, centrifuged, and treated with hydrogen peroxide to oxidize organic material. Phase contrast microscopy and ImageJ-based automated particle detection were used to quantify microplastic presence. Results showed significant particle reduction post-treatment, indicating successful removal of biological debris. South Lake Irvine had the highest particle concentration, while Rose Creek showed the greatest percentage decrease, suggesting high biological content. The method proved reliable across diverse sites and offers a standardized approach for environmental microplastic monitoring.

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