Unseen threats: Microplastic presence in waterpipe tobacco

Microplastics (MPs) are a ubiquitous form of waste and an emerging public health concern. MPs have been detected in various environmental matrixes, vegetables, and foods, but no data currently exists for tobacco samples. This research aimed to evaluate the concentration and characteristics of MPs in fresh tobaccos (fruit-flavored and regular) and their post-consumption waterpipe tobacco wastes (PWTWs). Our findings showed that the mean ± SD concentration of MPs particles in fresh flavored tobacco was 4.64 ± 1.59 items/g, whereas it was 0.79 ± 0.12 items/g in regular samples (p < 0.05). In the case of PWTW of flavored tobacco, the MPs concentration was 0.82 ± 0.07 items/g, while no particle was found in the PWTW of regular samples (p < 0.05). The most dominant size of MPs in fruit-flavored tobacco was 10–50 μm, while particles < 50μm (250–500μm) were the most frequent particles in fresh regular tobacco. Fibers were the most common shape among MP particles detected in both tobacco samples, with white/transparent and black being the predominant colors across all samples. The SEM-EDS (scanning electron microscope and energy-dispersive X-ray spectroscopy) analysis revealed that carbon and oxygen (C and O) were the primary elements detected in MPs present in waterpipe tobacco, confirming that these particles are plastic in nature. To better understand the implications of these findings, further research is needed to assess additional MP characteristics, such as polymer type. Moreover, investigating the potential toxicity of these MPs is crucial for understanding their effects on smokers' health. • Pioneer research on microplastics (MPs) in waterpipe tobacco. • Flavored tobacco displayed a notably greater number of MPs. • No MPs detected in post-consumption waste of regular tobacco. • Dominant MPs size shape were 10–50 μm and fibers, respectively. • SEM-EDS analysis certified MPs are plastic in nature.