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Mycorrhizal-specific responses of rhizosphere soil properties and fine-root traits to polystyrene microplastic addition in a temperate mixed forest

Communications Earth & Environment 2026
Yingtong Zhou, Yingtong Zhou, Ivano Brunner, Ziping Liu, Wei Guo, Wei Guo, Xiaoyue Na, Jiaxin Liu, Junni Wang, Junni Wang, Cunguo Wang, Mai-He Li

Summary

Researchers added polystyrene microplastics to a temperate forest and found they disrupted nutrient cycling differently depending on tree type — increasing nitrogen but decreasing phosphorus near oak-type trees, and doing the opposite near maple-type trees — suggesting microplastic pollution could reshape forest ecosystems over time.

Polymers

While microplastic impacts on aquatic and agricultural systems are well-documented, their impacts on forest ecosystems remain poorly understood. We assessed how microplastic addition affects rhizosphere soil properties and fine-root traits for ectomycorrhizal (ECM) and arbuscular mycorrhizal (AM) associations in a mixed temperate forest. In ECM-associated soils, microplastics increased nitrogen availability and nitrate reductase activity but decreased phosphorus and phosphatase activity; AM-associated soils showed the opposite pattern. Morphologically, ECM roots exhibited reduced branching but increased hyphal density and colonization. Conversely, AM roots displayed increased specific root length and tip density but decreased cortical thickness and tissue density. These divergent, mycorrhizal-specific responses suggest that increasing microplastic pollution may fundamentally alter nutrient cycling and species composition dynamics in temperate forests. After microplastic addition, rhizosphere nitrogen increased but phosphorus decreased in ectomycorrhizal trees, while arbuscular mycorrhizal trees showed an opposite trend of lower nitrogen and higher phosphorus in rhizosphere soil, based on a field study in Changbai Mountain Nature Reserve, China.

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