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The key role of magnetic iron-to-biochar mass ratios in the dissipation of oxytetracycline and its resistance genes in soils with and without biodegradable microplastics
Summary
Researchers synthesized magnetic biochars at different iron-to-biochar mass ratios and tested their ability to simultaneously remove the antibiotic oxytetracycline and its resistance genes from soils with and without PBAT biodegradable microplastics. A 1:2 iron-to-biochar ratio achieved the highest removal efficiencies (~95%) in both MPs-contaminated and clean soils, demonstrating biochar as a dual-action tool for antibiotic and resistance gene remediation.
There are challenges involved in the synergistic dissipation of antibiotics and antibiotic resistance genes (ARGs) in soil because ARGs are affected by not only the selective pressure of antibiotics but also microbial community succession and co-existing pollutants. Here, magnetic biochars (MBCs) at various magnetic iron-to-biochar mass ratios (3:1, 2:1, 1:1, 1:2, 1:3, 1:5, and 1:7) were synthesized to develop a strategy for the synergistic dissipation of oxytetracycline (OTC) and its resistance gene (tet) in soils with and without polybutylene adipate terephthalate (PBAT) microplastics (MPs). The results showed that MBC12 (1:2) achieved the greatest dissipation efficiencies of OTC in soils without and with PBAT MPs (95.27% and 94.50%, respectively). The reductive degradation of OTC via promoting the electron transfer during conversion between Fe(III) and Fe(II) overwhelmed biodegradation of OTC. MBCs effectively hindered the spread of tet in soil without PBAT MPs, with the efficiencies more than 60%; but they had little influence on its spread in soil with PBAT MPs, excluding MBC15 (1:5). The absolute abundance of tet, regardless of PBAT MPs, just significantly positively correlated with Serratia (the added exogenous tet-host bacterium), indicating that MBCs inhibited the horizontal transfer of tet at the inter-genus level. Down-regulating the degradation/utilization/assimilation metabolic function by MBCs (excluding MBC31, 3:1) contributed to the hindering class 1 integron gene (intI1)-driven tet propagation. After considering efficiency, cost and toxic effects, MBC12 (1:2) was recommended to use for synergistic dissipation of OTC and tet in soils without and with PBAT MPs.