Toxicological assessment of cigarette filter-derived microplastics in Daphnia magna

Cigarette filters are the most common form of litter worldwide and pose significant ecological risks because they degrade into microfibers and microplastics in aquatic environments. While previous studies have focused on the acute toxicity of cigarette leachate, the long-term ecological consequences of microplastic release from cigarette filters remain largely unexplored. This study evaluated the toxicity of cigarette filter-derived microplastics, including non-smoked cellulose acetate filters (CAF), smoked cigarette filters (GSF), on Daphnia magna, as well as leachate from smoked filter (LSF) for comparison. Imaging analysis confirmed that D. magna ingested cigarette filter-derived microplastics, which acted as carriers, gradually releasing harmful substances within organisms, a phenomenon consistent with the Trojan horse effect. Acute toxicity tests revealed similar 48-hour EC50 values (∼50 mg/L) for both GSF and LSF; however, GSF induced more pronounced long-term toxic effects. Chronic exposure to GSF significantly impairs reproduction, delays the timing of the first brood, reduces offspring size, and disrupts ecdysteroid-regulated genes. These findings indicate that cigarette filters are a persistent source of chemical pollution, threatening aquatic ecosystems. Specifically, microplastics from discarded cigarette filters act as Trojan horses, continuously releasing toxic chemicals and transporting hydrophobic contaminants, amplifying their environmental impact.