We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Study of acute lethality, teratogenesis, and metabolomic changes of N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) on Artemia franciscana
Summary
Researchers assessed the acute lethality, teratogenic potential, and metabolomic effects of a specific chemical compound in zebrafish embryos, using metabolomics to identify pathways disrupted at sublethal concentrations relevant to developmental toxicology.
Abstract The brine shrimp lethality test (Artemia spp.) is a classical model for assessing the toxicity of bioactive compounds. This study evaluated the toxicity and metabolomic changes induced by N-(2'- hydroxyphenyl)-2-propylpentanamide (HO-AAVPA), a derivative of valproic acid (VPA), in Artemia franciscana larvae using untargeted metabolomics through liquid chromatography–mass spectrometry (LC-MS/MS). The lethal concentration 50 (LC50) was determined by acute toxicity tests at 24 and 48 h, and teratogenic effects were assessed by measuring the larvae body length. Larval metabolomic changes were examined following 24- and 48-hour exposures to sublethal concentrations of HO-AAVPA (LC1=0.04 mM, LC10=0.2 mM) and VPA (LC1=1.79 mM, LC10=8.95 mM). After 48 h, HO-AAVPA had an LC50 of 0.32 mM, while VPA had 18.7 mM. VPA induced teratogenic effects at 9.6 mM; in contrast, HO-AAVPA only significantly affected the body length at 0.56 mM. Metabolomic analysis revealed that sublethal concentrations of HO-AAVPA affected the sphingolipid and glycerophospholipid metabolism, while VPA impacted alanine, aspartate, and glutamate metabolism. These findings suggest HO-AAVPA has high toxicity, but lower teratogenicity compared to VPA. In conclusion, the present study indicates that alterations in lipid and amino acid metabolism could be critical points in the mode of action of these compounds in A. franciscana.
Sign in to start a discussion.
More Papers Like This
An Integrated Metabolomics-Based Model, and Identification of Potential Biomarkers, of Perfluorooctane Sulfonic Acid Toxicity in Zebrafish Embryos
Researchers used advanced metabolomics techniques to study how the industrial chemical PFOS affects zebrafish embryo development at the molecular level. They identified specific metabolic disruptions and potential biomarkers that could indicate early PFOS exposure. The study provides new insights into how persistent environmental pollutants like PFAS interfere with biological processes during critical developmental stages.
Toxicological Assessment of Ketamine in Juvenile Zebrafish (Danio rerio)
Researchers studied how ketamine exposure affects juvenile zebrafish behavior and metabolism, finding significant changes in swimming activity and metabolic profiles at various concentrations. While primarily focused on pharmaceutical contamination, the study used methods relevant to understanding how emerging contaminants in waterways affect aquatic life. The research highlights the value of zebrafish as a model for studying how pollutants in water bodies impact developing organisms.
Transcriptome analysis reveals hepatotoxicity in zebrafish induced by cyhalofop-butyl
This study investigated hepatotoxicity in zebrafish induced by the herbicide cyhalofop-butyl using transcriptome analysis to identify affected biological pathways. Note: this preprint was withdrawn by bioRxiv due to concerns about manuscript validity and author identity.
Embryotoxic and teratogenic effects of polyethylene microbeads found in facial wash products in Zebrafish ( Danio rerio) using the Fish Embryo Acute Toxicity Test
This study used standardized zebrafish embryo toxicity testing to evaluate whether polyethylene microbeads from facial cleansers cause developmental abnormalities, finding dose-dependent mortality and multiple teratogenic effects including skeletal deformities. Cosmetic microbeads that enter waterways pose genuine developmental risks to aquatic vertebrates.
Metabolic Consequences of Developmental Exposure to Polystyrene Nanoplastics, the Flame Retardant BDE-47 and Their Combination in Zebrafish
Researchers examined how developmental exposure to polystyrene nanoplastics and the flame retardant BDE-47, alone and combined, affects zebrafish metabolism, finding that co-exposure produced distinct metabolic disruptions beyond those caused by either contaminant individually.