Microplastic contamination in sediments: Analytical techniques and case-based evaluations

Microplastics (MPs) pollution in sediments has gained critical attention due to its pervasive presence and potential ecological risks. This review synthesizes the latest advancements in analytical techniques, providing a comprehensive overview of separation and identification methods tailored to complex sedimentary matrices. Density-based approaches, such as ZnCl or NaI solutions, and enzymatic digestions are increasingly refined to isolate MPs of varying sizes, yet discrepancies in mesh sizes, reagent concentrations, and digestion protocols continue to complicate cross-study comparisons. Meanwhile, cutting-edge spectroscopic tools-μFTIR, Raman imaging, thermal analyses-have greatly enhanced polymer identification down to the tens-of-micrometers scale. Case studies spanning urban estuaries to remote deep-sea basins underscore the pervasive nature of MPs worldwide, with fibers and fragments frequently dominating sediment samples. Factors such as polymer density, hydrodynamics, and biofouling contribute to the diverse distribution patterns, revealing that even ostensibly pristine environments are not exempt from contamination. Although the precise ecological and toxicological consequences of long-term sediment-bound MPs remain partly unclear, growing evidence points to intricate interactions with co-occurring contaminants and potential trophic transfer. To address these knowledge gaps, this review emphasizes the urgent need for methodological standardization and collaborative initiatives, particularly for emerging challenges like nanoplastic detection. By integrating robust sampling approaches, advanced analytical tools, and interdisciplinary research, scientists and policymakers can more accurately map and mitigate the impacts of sediment-associated MPs on aquatic ecosystems.