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High-throughput microplastic screening system using multispectral fluorescence imaging under UV excitation

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Researchers tested a faster way to identify microplastics by shining UV light on samples and measuring the glow each plastic type gives off, rather than using slower standard lab methods. Using a small set of just 5 light measurements and a computer analysis method, the system correctly identified 9 common plastic types, suggesting simpler, cheaper equipment could screen for microplastics more quickly.

The widespread contamination of microplastics (MPs) necessitates comprehensive monitoring across various environments. However, standard analytical methods, such as FTIR and Raman spectroscopy, rely on point- scanning, which is time-consuming and resource-intensive, limiting their utility for high-throughput screening. Here, we propose a rapid multispectral imaging method using polymer auto-fluorescence under UV excitation. Leveraging the distinct fingerprints observed in Excitation-Emission Matrices (EEMs), our approach optimizes spectral acquisition by selecting a minimal subset of effective spectral bands rather than measuring the full set of spectra in the EEM. Using a Random Forest-based feature selection, we demonstrated that a subset of 5 spectral bands maximizes the classification performance for 9 representative virgin polymers, achieving a weighted F1-score of 0.92. This significant reduction in spectral dimensionality enables the design of simplified hardware using commercially available UV-LEDs, offering a practical and accessible solution for high-throughput MP monitoring.

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