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Microplastics in freshwater environments across the high altitude region of Gilgit-Baltistan, Pakistan

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Even remote, high-altitude rivers and lakes in northern Pakistan contain tiny plastic particles, found in water, sediment, and fish. Farmed fish had the most microplastics, followed by market fish, while wild fish had the least, suggesting farming practices add extra plastic exposure. Since these fish are eaten locally, this raises questions about how much plastic ends up on people's plates.

Polymers
Body Systems
Study Type Environmental

Microplastics are increasingly recognized as pervasive contaminants in remote mountain freshwater ecosystems, yet evidence from high-altitude regions remains limited. This study investigated microplastics occurrence and characteristics in surface water, sediments, and fish from the Braldu-Shigar-Skardu freshwater system in Gilgit-Baltistan, northern Pakistan. Samples were collected from remote headwaters, tributaries, main river channels, and the Satpara Lake-Dam system. Fish originated from wild populations, farming facilities, and local markets to provide a broader assessment of microplastic exposure. Matrix-specific extraction protocols were applied, followed by stereomicroscope sorting and polymer identification by µFT-IR spectroscopy. Microplastics were detected in nearly all sites and matrices, with concentrations ranging from 1 to 17 MPs L -1 in water, 55–537 MPs kg -1 in sediments, and 1–11 MPs individual -1 in fish gastrointestinal tracts. Notably, highest frequency of microplastics occurrence was measured in farmed individuals (78%–83%), suggesting that aquaculture-related practices may represent an additional source of microplastics. Wild fish showed the lowest occurrence (25%), whereas those obtained from local markets revealed intermediate values (62%), indicating variable exposure across different pathways. Surface waters were dominated by small fragments and polypropylene, indicative of recent inputs and origin from fragmentation. Sediments showed a broader size distribution, and greater polymer diversity, consistent with their role as long-term sinks. The highest polymer diversity was highlighted in fish, indicating multiple exposure pathways. Spatial patterns did not show a geographical downstream increase, but rather a patchy distribution shaped by local anthropogenic input, hydrological connectivity, and depositional dynamics. Glacier-fed rivers primarily act as transport corridors, whereas low-energy environments such as Satpara Lake function as sinks promoting microplastics accumulation. Overall, the study provides the first integrated multi-compartment baseline for microplastics in Gilgit-Baltistan and supports future monitoring and management actions for these high altitude critical freshwater resources.

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