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Original immunofluorescence images and quantification data supporting PI3K-Akt pathway analysis in microplastic-exposed DSS colitis
Summary
Scientists studying mice with colitis (a type of inflammatory bowel disease) found that exposure to microplastics ramped up activity in a cell signaling pathway (called PI3K-Akt) that's linked to inflammation and disease severity. This suggests microplastics might make gut inflammation worse, though this study only shares the underlying image data and measurements, it doesn't pinpoint exactly which gut cells are affected, so more research is needed before drawing firm conclusions about human health risks.
This dataset contains the original immunofluorescence TIFF exports and replicate-level quantification data supporting Figure 9, Supplementary Figure 8, and Supplementary Tables 1–2 of the related manuscript, “Microplastic exposure activates PI3K-Akt signaling in ulcerative colitis: integrated computational toxicology, RNA-seq, GeneMANIA network analysis, and in vivo validation.” The dataset includes p-AKT and p-PI3K staining of mouse colonic tissue from the normal control, DSS-colitis, and DSS-colitis + microplastics groups. For each marker and group, three biologically independent samples are provided. Each sample contains a DAPI image, a red target-channel image, and the corresponding merged image, yielding 54 original TIFF files. All TIFFs are 1920 × 980 pixels and contain 25-µm scale bars. The files were copied and renamed without pixel modification. A manifest links every deposited image to its publication panel, experimental group, biological replicate, fluorescence channel, original source filename, and SHA-256 checksum. Replicate-level Image-Pro Plus measurements and normalized-density values are also included. The p-AKT staining pattern was interpreted as epithelial-predominant based on tissue morphology. Because no epithelial cell-type-specific co-staining was performed, these images do not establish definitive cell-type localization.