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Influence of pH and protein concentration on the development of myofibrillar protein-based biodegradable film from Basa (Pangasius bocourti) fish waste

Frontiers in Nutrition 2026
Prashant K. Mishra, Mohd Danish, Vivekanand Kumar, Aarti S. Kakatkar, Raj Kamal Gautam, Suchandra Chatterjee

Summary

Scientists turned leftover parts of Basa fish (usually thrown away by the seafood industry) into a biodegradable food packaging film, tackling two problems at once: fish waste pollution and plastic waste. By tweaking the acidity and protein levels during production, they found a recipe that created a stronger, more water-resistant film than other versions tested, suggesting a promising path toward packaging that breaks down naturally instead of persisting in the environment like conventional plastic, which sheds microplastics into our food, water, and bodies.

Fish waste generated by fish-processing industries contributes significantly to environmental pollution, highlighting the need for its effective utilization. Similarly, plastic waste from packaging industries poses serious environmental and health concerns. In this study, a biodegradable film was developed using myofibrillar protein obtained from Basa ( Pangasius bocourti ) fish waste. Protein concentrates at different concentrations (0.5, 1.0, and 1.5%) were used to prepare films under two pH conditions (2.8 and 11.0). The films were evaluated for their tensile strength, elongation at break, water vapor permeability, water solubility, and color characteristics. Among the formulations tested, the film prepared with 1% protein concentrate at pH 2.8 exhibited the highest tensile strength (6.86 MPa), the lowest water solubility (28.1%), and a lower increase in yellowness during storage compared to the other films. Films developed under acidic conditions showed greater disulphide interactions and a smoother surface morphology than those prepared under alkaline conditions. Thus, a myofibrillar protein-based biodegradable film was developed and it was systematically demonstrated how pH and protein concentration affect their mechanical, barrier, and physicochemical properties. The innovation of this work lies in integrating Basa fish waste valorization with pH-mediated protein engineering to develop biodegradable films.

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