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Genetically Engineered Algae to Degrade Plastic

ScholarSpace - JCCC (Johnson County Community College) 2026
Xavier Seurer, Isaac Duckworth

Summary

Scientists tried to genetically modify a common algae (chlorella) to produce enzymes that break down plastic, which could eventually help reduce the plastic pollution that ends up as microplastics in our water, food, and bodies. This initial attempt to insert the plastic-degrading genes didn't work as planned, so the approach needs more refinement before it could become a real tool against plastic waste. It's an early, unsuccessful step in ongoing research—not a breakthrough yet—but it points toward a promising direction for tackling plastic pollution's impact on ecosystems and human health.

Plastic pollution is a severe global issue that effects multiple aspects of the world. The ecosystem, wildlife and even human health are all majorly affected by the amount of plastic waste. The end goal is to engineer a wildlife chlorella into an algae that produces specific enzymes that break down plastics. To accomplish this, a specific part of our DNA will be introduced to the algae via electroporation. To see if the incorporation was successful, we will perform multiple selection processes to verify and confirm that the electroporation was successful. After selecting a couple colonies of our algae sample, a screening will be done on the clones to further ensure that the DNA and proteins for plastic degradation are present. Once verified the DNA will then be separated in order to measure the concentration and a PCR reaction will then be conducted. The electroporation process was overall unsuccessful in introducing our DNA to the wildlife chlorella. When the Algae was introduced to hygromycin, little to none survived. A sample collected from a peer was used instead for further analysis. The DNA in this sample was fortunately positive. Plastic pollution continues to effect key and crucial aspects of this world to this day, but by successfully engineering wildlife chlorella to produce these enzymes, plastics will cause a much less impact on the ecosystem, wildlife, and our health going forward.

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