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Asynchronous dual-Lissajous scanning for shuffle-coded compressive Raman imaging
Summary
Scientists developed a faster, gentler way to scan cells and materials with laser light to identify their chemical makeup, without damaging delicate samples like living cells. This new scanning method was tested successfully on microplastics and yeast cells, meaning it could help researchers better study how microplastics interact with living cells—an important step in understanding what these tiny plastic particles might mean for our health.
We present an asynchronous Lissajous scanning strategy that independently controls the excitation and scattering projection paths, enabling shuffle‑coded compressive confocal Raman imaging. This scanning strategy projects scattered photons onto the spectrometer entrance in a randomly interleaved manner without sacrificing scanning speed. In contrast to the step-and-settle scanning used in earlier SIRI implementations, the continuous Lissajous trajectories enable seamless spatial sampling with a substantially reduced dwell time of the focused laser spot at each sampling point, thereby mitigating photobleaching and photodamage. Furthermore, compared to single-Lissajous scanning of laser beam for undersampling-based compressive hyperspectral imaging, the dual-Lissajous approach achieves significantly improved reconstruction fidelity in both spatial and spectral domains. The feasibility of the proposed method is demonstrated through numerical simulations and experimental studies involving microplastics and single yeast cells.