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Emerging Analytical Methodologies for Micro- and Nano-Plastics Detection in Human Samples: Analytical Challenges and Health Risk Perspectives
Summary
Scientists have found tiny plastic particles (microplastics and even smaller nanoplastics) throughout human organs and body fluids, but this review of existing research shows we still lack reliable, standardized tools to accurately measure them—different labs get different results depending on how they prepare and test samples. While lab studies suggest very small plastic particles can cause cell stress and inflammation, there isn't yet solid evidence directly linking plastic exposure to specific diseases in people—so the biggest takeaway is that we urgently need better testing methods before we can truly understand the health risks these particles pose.
Micro- and nanoplastics (MNPs) are increasingly detected in human tissues and biofluids, raising concerns about their potential health impacts. Yet, despite growing evidence of human exposure, reliable characterization of these particles remains a major analytical challenge. This review critically evaluates sample-preparation strategies, detection methodologies, and toxicological evidence for MNPs in human biological samples. MNPs have been detected across major human organ systems and biofluids. Sample preparation strongly affects MNP analytical accuracy and inter-study comparability; harsh digestion reduces matrix interference but may degrade polymers, whereas milder treatments preserve particles but leave organic residues. These methodological tradeoffs contribute substantially to variability among studies and complicate cross-study comparisons. Although experimental evidence indicates that particles smaller than 10 µm can induce oxidative stress, inflammation, and cellular dysfunction, current epidemiological evidence linking MNP exposure to human disease remains largely correlational. Comparative assessment of microscopy-based approaches, vibrational spectroscopy, and pyrolysis-gas chromatography-mass spectrometry reveals that no single analytical technique can simultaneously provide accurate particle sizing, polymer identification, and mass quantification. We highlight critical limitations of various techniques, including quantification, morphological information, resolution, and the potential for false-positive polymer assignments arising from sample preparation methodologies, residual biological matrices, and their pyrolysis products. Furthermore, nanoplastics, the fraction considered most biologically relevant, remain severely under-quantified because their dimensions fall below the practical detection limits of most routine analytical methods. Standardized protocols, certified reference materials, validated nanoplastic detection methods, and integrated multi-analytical detection strategies are urgently needed to advance reliable MNPs biomonitoring, improve inter-study comparability, and establish robust exposure-risk assessments.