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Classification, Quantification, and Fluorescence Behavior Analysis of Microplastics via Nile Red Staining: Application to Detection Emission in Takeaway Food Container
Summary
Researchers optimized Nile Red staining protocols using hexane-acetone solvent mixtures to fluorescently classify and quantify microplastics by polymer type, then applied the method to seven takeaway food containers, finding 420–1,326 MPs per box—predominantly 10–20 µm irregular fragments—with SEM revealing surface cracking that drives particle release.
-hexane 3:1 (v/v), 10 μg/mL, immersion staining) achieves high FI, successfully differentiates among all polymers. Seven takeaway containers released 420-1326 MPs/box, most of which were between 10 and 20 μm and in irregular fragments. SEM observations confirmed that exposures induced surface cracks and wrinkles, which significantly compromised surface integrity and facilitated the release of MPs.