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Classification, Quantification, and Fluorescence Behavior Analysis of Microplastics via Nile Red Staining: Application to Detection Emission in Takeaway Food Container
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Researchers optimized Nile Red staining protocols using hexane-acetone solvent mixtures to fluorescently classify and quantify microplastics by polymer type, then applied the method to seven takeaway food containers, finding 420–1,326 MPs per box—predominantly 10–20 µm irregular fragments—with SEM revealing surface cracking that drives particle release.
-hexane 3:1 (v/v), 10 μg/mL, immersion staining) achieves high FI, successfully differentiates among all polymers. Seven takeaway containers released 420-1326 MPs/box, most of which were between 10 and 20 μm and in irregular fragments. SEM observations confirmed that exposures induced surface cracks and wrinkles, which significantly compromised surface integrity and facilitated the release of MPs.
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Dyeing to Know: Optimizing Solvents for Nile Red Fluorescence in Microplastics Analysis
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Researchers investigated how the choice of solvent affects Nile Red fluorescence staining for microplastic identification, optimizing solvent conditions to improve the reliability of fluorescence-based classification of microplastic polymer types in environmental samples.
Nile Red Staining as a Subsidiary Method for Microplastic Quantifica-tion: A Comparison of Three Solvents and Factors Influencing Application Reliability
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Researchers compared three solvents — acetone, chloroform, and n-hexane — for Nile Red fluorescent staining of microplastics across multiple polymer types and post-consumer materials, finding chloroform most effective with an 83.3% recovery rate, though the method falls short of the reliability of Raman or FTIR spectroscopy.
Nile Red Staining as a Subsidiary Method for Microplastic Quantifica-tion: A Comparison of Three Solvents and Factors Influencing Application Reliability
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This study evaluated Nile Red fluorescent staining as a method for quantifying microplastics in environmental samples, comparing it to traditional identification techniques. The approach can help distinguish microplastics from organic particles more quickly and cost-effectively, supporting higher-throughput microplastic analysis in environmental monitoring programs.
Identification and quantification of microplastics using Nile Red staining
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Researchers tested Nile Red staining as a method for identifying and quantifying microplastics in environmental samples, finding it useful for rapid screening but noting limitations in distinguishing plastics from non-plastic particles.
Quantification of microplastics by count, size and morphology in beverage containers using Nile Red and ImageJ
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Microplastics in beverage containers were quantified by count, size, and morphology to characterize a potential route of human exposure to these particles. The study found microplastics across beverage container types, providing controlled quantitative data on how many particles consumers may ingest through drinks.
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