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Assessment of Different Sampling, Sample Preparation and Analysis Methods Addressing Microplastic Concentration and Transport in Medium and Large Rivers Based on Research in the Danube River Basin
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Monitoring microplastics in rivers is hampered by the lack of standardized methods, making it difficult to compare results across studies. This research tested three common sampling approaches on the Danube River and its tributaries, finding that each method produced meaningfully different estimates of microplastic concentrations and transport. The results underscore the urgent need for agreed-upon protocols so that data from different countries and research groups can be reliably combined to track river-to-ocean plastic pollution.
Since the introduction of the Sustainable Development Goals, and, in particular, with the goal of reducing marine pollution (SDG 14.1), riverine microplastics are attracting public and scientific attention. But standardized monitoring methods and comparable data are still missing. Therefore, the opportunity was taken to test three of the most common monitoring methods (multiple depths net-method, pressurized fractionated filtration and sedimentation-box) at seven sites in five countries along the Danube and the Tisza Rivers. Different boundary conditions (hydrological and morphological conditions, economic situation, equipment available, etc.) were considered for the evaluation, as well as different sampling methods and sample pre-treatments together with different methodologies for microplastic identification. The sampling methods were evaluated for their suitability to be used as a standard monitoring tool in the future. Only net sampling and pressurized fractionated filtration allow for the determination of microplastic concentration as well as load, and can therefore be recommended. The multi-depth net device, as a labor-intensive method, is recommended if the focus of the monitoring is on larger particles and it is important to calculate particle and mass concentrations. Pressurized fractionated filtration is a practical tool recommended for routine monitoring, having the advantage of less effort being required for sample preparation and simply considering small particle sizes below 500 µm. From a scientific perspective it is recommended to combine both the pump sampling and the net-based device.
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