Genové úpravy sinic pro degradaci plastů

Modern times are struggling with a lot of global problems, one of them is plastic waste. Many types of microorganisms capable of biodegrading microplastics have been investi-gated, but most of them are bacteria. Unlike cyanobacteria or microalgae, bacteria have a higher efficiency of biodegradation of microplastics, but this disadvantage gives these microorganisms the potential to improve their biodegradation ability, and gene modifi-cation is one of the possible means to achieve this. However, marine micro-organisms such as cyanobacteria or microalgae remain neglected in studies. For that reason, the cyanobacteria, freshwater Synechococcus elongatus PCC 7942, Nostoc sp. PCC 7120 (An-abaena sp.) and marine Synechococcus sp. PCC 11901 selected as representatives for genetic modification with synthetically assembled PETase and PSase (serine hydrolase) genes. Genetic modification was carried out by the method of conjugation and classical transformation. In the cyanobacteria Synechococcus elongatus PCC 7942, Nostoc sp. PCC 7120 conjugation was successful and exconjugant formation was demonstrated on medi-um with antibiotics. The classical transformation performed in the cyanobacteria Nostoc sp. PCC 7120 and Synechococcus sp. PCC 11901 was negative. For future experiments, it is necessary to verify the presence of plasmids in cyanobacterial exconjugants and per-form further studies on the efficiency of enzyme expression, transgenic plasmid mainte-nance and cultivation with microplastic.